FIGURE 5.

ASAP2 promotes cell migration and positively regulates the EGFR/ERK signaling pathway in PDAC cells. A, Wound healing assays using ASAP2 knockout Panc1 and MiaPaCa2 cells. The migrated distance was quantified by measuring the difference at 0, 24, and 48 h and was normalized to 0 h. n.s. not significant; (*) P < .05; (**) P < .01; (***) P < .001. B, Wound healing assays using MiaPaCa2 cells stably overexpressing ASAP2. The migration distance was quantified by measuring the difference at 0, 24, and 48 h and was normalized to 0 h. n.s. not significant; (***) P < .001. C, ASAP2 overexpressing or control MiaPaCa2 cells and ASAP2 knockout or wild‐type Panc1 and MiaPaCa2 cells were analyzed for levels of phosphorylated or total EGFR and ERK using western blotting. β‐Actin was used as the loading control for relative protein quantification. The normalized intensities of each protein and ratios of pEGFR/EGFR and pERK/ERK are shown