FIGURE 2.

circ_100984 silencing repressed BC cell proliferation, migration, invasion, and EMT in vitro and in vivo. A, Knockdown efficiencies of sh‐circ_100984‐1 and sh‐circ_100984‐2 were examined by qRT‐PCR in Biu‐87 and HTB9 cells. B, After 0, 1, 2 and 3 d of transfection with sh‐NC, sh‐circ_100984‐1 and sh‐circ_100984‐2, Biu‐87, and HTB9 cells were subjected to cell viability analysis using CCK‐8 assay. C, Colony formation assay was performed to assess the proliferation of Biu‐87 and HTB9 cells after transfection with sh‐NC, sh‐circ_100984‐1 and sh‐circ_100984‐2. Effects of circ_100984 knockdown on cell (D) migration and (E) invasion were evaluated by wound‐healing and transwell assay, respectively. F, Immunofluorescence comparing circ_100984 silenced Biu‐87 and HTB9 cells with their respective cells are shown for relative expression of Vimentin and E‐cadherin. G, Protein expression levels of YBX‐1, Slug‐1, Vimentin, N‐cadherin, and E‐cadherin in the tumors of nude mice following sh‐NC, sh‐circ_100984‐1 and sh‐circ_100984‐2 transfection were examined by western blot. *P < .05; **P < .01; ***P < .001