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Optimization of the CHA reaction.
(A) Optimization of the H2 concentration (50–200 nM) at a constant H1 concentration (50 nM) in a 2 h reaction at 50 °C; (B) Reaction temperature optimization using a 2 h reaction with 50 nM of H1 and 150 nM of H2; (C) Optimization of the CHA reaction time at 50 °C with 50 nM of H1 and 150 nM of H2; (D) Optimization of the concentrations of H1 and H2 in a 30 min reaction at 50 °C.
