Table 1.

Comparison of CHA-LFIA and RT-PCR for SARS-CoV-2 RNA reference material detection.

Copies/mL	RT-PCR			CHA-LFIA fluorescence values
	Result	Ct Value		Orf1ab			N
		Orf1ab	N	Test 1	Test 2	Test 3	Test 1	Test 2	Test 3
500,000	Positive	18.25	17.33	11,960	8950	8997	9463	8215	10,448
100,000	Positive	20.43	18.38	5669	4430	5401	4688	5805	3752
20,000	Positive	21.68	19.99	2319	2800	1921	1707	1695	2725
4000	Positive	29.62	28.50	892	598	734	523	817	825
800	Positive	36.59	35.63	192	131	72	167	100	198
160	Negative	39.16	38.32	180	125	123	102	99	186
0	Negative	0	0	95	69	79	93	133	140