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. 2021 Mar 31;9:e10890. doi: 10.7717/peerj.10890

Figure 4. Immunofluorescence staining of urothelial markers at the cellular level.

Figure 4

(A) ADSCs cultured in CM and different concentrations of pHPL (5% & 2.5%) or with FBS, were assessed for the expression of cytokeratin-18 (FITC, green) and uroplakin- 2 (Cy3, red). SV-HUC cells were used as a positive control, meanwhile ADSCs were utilized as a negative control. Scale bar = 50 µm. (B & C) Semi-quantitative analysis of immunoflouresence representing percentage of positive cells relative to negative control. All experiments were repeated at least three times.