Table 1. High-throughput sequencing outcomes for regular and nested PCRs.

Samples consist of the 12 samples described in the collection methods plus two negative and two positive controls. Each was subjected to two PCR procedures (PCR 3&4). Values represent the total number of sequences from each sample assigned to mole-rat and human targets and false positive assignments to dog and sheep, along with sample time, source and filter used to collect the air.

Sample	Sample time	Source	Filter	Mole rat	Human	Cow	Dog	Sheep
1	5	Burrow System	0.22	5,905	836	0	0	0
2	5	Facility Room	0.22	43	12,836	0	0	0
3	5	Burrow System	0.45	6,689	5,196	0	44	0
4	5	Facility Room	0.45	881	5,637	0	344	0
5	10	Burrow System	0.22	4,318	8,734	0	0	0
6	10	Facility Room	0.22	655	8,381	0	1,866	0
7	10	Burrow System	0.45	1,601	2,787	0	0	0
8	10	Facility Room	0.45	530	4,816	0	0	0
9	20	Facility Room	0.45	0	2,534	0	0	0
10	20	Facility Room	0.22	0	8,953	0	0	0
11	20	Burrow System	0.45	8,268	2,255	0	0	0
12	20	Burrow System	0.22	7,756	6,998	0	0	0
Neg 1	NA	NA	NA	0	5,756	0	3,408	0
Neg 2	NA	NA	NA	0	4,186	0	0	0
Pos 1	NA	NA	NA	0	13	21,756	0	91
Pos 2	NA	NA	NA	0	0	16,692	0	96
Nested_1	5	Burrow System	0.22	11,904	7393	0	0	0
Nested_2	5	Facility Room	0.22	0	8,458	0	0	0
Nested_3	5	Burrow System	0.45	10,160	7,561	0	0	0
Nested_4	5	Facility Room	0.45	0	17,713	0	0	0
Nested_5	10	Burrow System	0.22	7,643	11024	0	0	0
Nested_6	10	Facility Room	0.22	0	10,813	0	6,550	0
Nested_7	10	Burrow System	0.45	0	19,363	0	0	0
Nested_8	10	Facility Room	0.45	0	18,289	0	0	0
Nested_9	20	Facility Room	0.45	0	12,219	0	0	0
Nested_10	20	Facility Room	0.22	1,724	10,426	0	674	0
Nested_11	20	Burrow System	0.45	9,075	6,710	0	0	0
Nested_12	20	Burrow System	0.22	7,198	6,786	0	0	0
Nested_Neg 1	NA	NA	NA	0	0	0	0	0
Nested_Neg 2	NA	NA	NA	0	17,146	0	0	0
Nested_Pos 1	NA	NA	NA	0	16	12,390	0	116
Nested_Pos 2	NA	NA	NA	0	65	14,817	0	119