FIG. 5.
The effects of CS and VEGF (50 ng/ml) on morphology of the vasculature formed by HUVECs. For each experimental condition, images of the whole vessel showing F-actin staining are shown. For better visualization of the vascular morphology, the overlay of the F-actin (red), PECAM-1 (green), and DAPI (blue) staining is shown as a close-up view. Regardless of the treatment with VEGF, CS resulted in HUVEC actin re-organization, cellular elongation, and increasing vascular stability.