Table 2.
Primers sequences used for quantitative RT-PCR.
| Gene | Primer sequences (5′-3′) | Product length, bp |
|---|---|---|
| Nrf2 | F: GCCCCTGGAAGCGTTAAAC | 67 |
| R: GGACTGTATCCCCAGAAGGTTGT | ||
| HO-1 | F: CGCTCCCGAATGAACAC | 112 |
| R: GCTCCTGCACCTCCTC | ||
| TNF-α | F: TCTCATGCACCACCATCAAGGACT | 178 |
| R: ACCACTCTCCCTTTGCAGAACTCA | ||
| IL-1β | F: ACCTGTGTCTTTCCCGTGG | 92 |
| R: TCATCTCGGAGCCTGTAGTG | ||
| IL-6 | F: ATCCAGTTGCCTTCTTGGGACTGA | 162 |
| R: TAAGCCTCCGACTTGTGAAGTGGT | ||
| β-Actin | F: TGGAA CGGTG AAGGT GACAGC | 177 |
| R:GCTTTTGGGAA GGCAG GGACT | ||
| NF-κB | F: CACTGTCACCTGGAAGCAGAG | 139 |
| R: CACACATCTCCTTTCTCATTGC | ||
| TLR-4 | F: TTACAGAAGCTGGTTGCCGT | 152 |
| R: TCCAGGTTGGGCAGGTTAGA |
TLR4: Toll-like receptor 4; NF-κB: nuclear factor-κB; Nrf2: nuclear factor erythroid-derived 2-related factor 2; HO-1: heme oxygenase-1; interleukin-1β: IL-1β; IL-6: interleukin-6; TNF-α: tumor necrosis factor-α.