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. 2021 Mar 27;2021:5896931. doi: 10.1155/2021/5896931

Figure 4.

Figure 4

HO-1 expression was elevated in DOX-induced H9c2 cells treated with XML. (a) Real-time PCR of HO-1 was represented with normalization to GAPDH and β-actin. (b, c) Western blotting of HO-1 was represented with normalization to β-actin. H9c2 cells were treated with 5 μmol/L DOX only or preincubated with 1 mg/mL XML for 48 h. (d) Real-time PCR of HO-1 was represented with normalization to GAPDH. Data are representative of at least three independent experiments (mean ± SD). Statistical significance was assigned as ∗P < 0.05 and ∗∗P < 0.01 compared with control and ##P < 0.01 compared with DOX. P values were determined using the independent one-way ANOVA and the Tukey-Kramer test. XML: Xinmailong treatment; DOX: doxorubicin.