Figure 6.

Downregulation of HO-1 abrogates the lysosome functions of XML in DOX-induced H9c2 cells. (a) Lysosome was labeled by LysoTracker Red staining. (b) Lysosomal pH was determined by using fluorescein-dextran. H9c2 cells were treated with 5 μmol/L DOX for 24 h only or preincubated with 1 mg/mL XML for 48 h. Data are representative of at least three independent experiments (mean ± SD). Statistical significance was assigned as ^∗P < 0.05 and ^∗∗P < 0.01 compared with DOX and ^#P < 0.05 compared with DOX+XML. P values were determined using the independent one-way ANOVA and the Tukey-Kramer test. XML: Xinmailong treatment; DOX: doxorubicin.