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. 2021 Mar 30;14:2279–2291. doi: 10.2147/OTT.S282947

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© 2021 Yang et al.

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Silencing of LINC00460 attenuated HNSCC cell proliferation, invasion, migration, and EMT. (A) RT-qPCR analysis of LINC00460 mRNA in Cal-27 and FaDu cells treated with sh-LINC00460 (B) CCK-8 assays were applied to detect cell viability of Cal-27 and FaDu cells. (C) Colony formation assays were used to measure cell proliferation ability. (D and E) Representative results of transwell migration assays and invasion assays in Cal-27 and FaDu cells. (F) Relative expression of EMT-related genes in Cal-27 and FaDu cells detected by Western blot analysis. (scale bar: 200 μm for transwell assay). **P < 0.01, ***P < 0.001.