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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Biol Psychiatry. 2020 Oct 5:S0006-3223(20)31952-1. doi: 10.1016/j.biopsych.2020.09.022

Figure 1: Outline of workflow for TRAP sequencing to reveal sex differences in nociceptor translatomes.

Figure 1:

A, eGFP-L10a protein is expressed in Nav1.8-positive nociceptors. B, Schematic representation of the methodology shows dissection of all DRGs (cervical, thoracic and lumbar) from Nav1.8cre/Rosa26fsTRAP mice followed by isolation of total RNA (INPUT), and mRNA-bound to the ribosome (IP) using anti-eGFP-coated beads; Samples were sequenced and processed for downstream analysis of differentially expressed genes as shown.