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. Author manuscript; available in PMC: 2023 Jan 1.
Published in final edited form as: Biol Psychiatry. 2020 Oct 5:S0006-3223(20)31952-1. doi: 10.1016/j.biopsych.2020.09.022

Figure 7: Intraplantar administration of PGE2 produces greater mechanical allodynia and grimacing in female mice.

Figure 7:

A, Male mice did not respond to von Frey filaments after injection of 300 ng (Two-way ANOVA RM, F = 1.030, p-value = 0 .3900) or 1 μg (Two-way ANOVA RM, F = 0.7260, p-value = 0.5444) of PGE2. When calculating the effect size we did not observe any statistical significant differences between groups in males (Effect size 300 ng: Unpaired t-test, t = 0.8756, df = 13, p-value = 0.3971; Effect size 1 μg: Unpaired t-test, t = 0.8709, df = 10, p-value = 0.4042). B, Female mice showed mechanical allodynia up to 24 hours after injection of both 300 ng of PGE2 (Two-way ANOVA, F = 12.35, p-value <0.0001, post-hoc Sidak’s: * Vehicle - 300ng PGE2 at 4h, p-value = 0.0161, **** Vehicle - 300ng PGE2 at 24h, p-value <0.0001) and 1 μg of PGE2 (Two-way ANOVA RM, F = 10.78, p-value <0.0001, post-hoc Sidak’s: *** Vehicle - 1 μg PGE2 at 4h, p-value = 0.0005, ** Vehicle - 1 μg PGE2 at 24h, p-value = 0.0026). We also observed an effect size difference between groups in female mice (*** Effect size 300 ng: Unpaired t-test, t = 4.980, df = 13, p-value = 0.0003; *** Effect size 1 μg: Unpaired t-test, t = 5.569, df = 10, p-value = 0.0002). C, Male mice did not show any significant grimacing behaviors following administration of 300 ng of PGE2 (Two-way ANOVA RM, F = 1.996, p-value = 0.1305). At 1 μg of PGE2 we also did not observe any significant grimacing in male mice (Two-way ANOVA RM, F = 0.5376, p-value = 0.6601). Similarly, we also do not observe any effect size between groups in grimacing of male mice (Effect size 300 ng: Unpaired t-test, t = 0.9225, df = 13, p-value = 0.3731; Effect size 1 μg: Unpaired t-test, t = 0.8305, df = 10, p-value = 0.4257). D, Female mice exhibit robust grimacing after 1 μg PGE2 injection (Two-way ANOVA RM, F = 11.34, p-value <0.0001, post-hoc Sidak’s: **** Vehicle - 1ug PGE2 at 30 min, p-value<0.0001, * Vehicle - 1ug PGE2 at 60 min, p-value = 0.0257) but not at 300 ng (Two-way ANOVA RM, F = 1.197, p-value = 0.1303). We also observed an effect size in the grimacing scores between 1 μg PGE2 and vehicle grimacing in female mice (Effect size 300 ng: Unpaired t-test, t = 0.9458, df = 13, p-value = 0.3615; *** Effect size 1 μg: Unpaired t-test, t = 4.669, df = 10, p-value = 0.0009).