Figure 3.

Inhibition of autophagy during elastin challenge alleviates the neutrophilic airway inflammation in vivo. (A) Experimental outline and protocol for 3-MA treatment, 3-MA (4 mg/ml, 50 μl) was administrated intratracheally 2 hrs after each elastin peptide (2 mg/ml, 50 μl) challenge. (B) Inflammatory cell counts in the BALF. (C–F) The Cxcl1, Cxcl2, Il6 and Ifng mRNA transcripts in lungs. Labeling for all the columns throughout Figure 3 was shown in (B). Mac, macrophages; Neu, neutrophils; Lym, lymphocytes; Eos, eosinophils. Throughout, data are representative of 4-5 mice and were replicated in at least 3 independent experiments. Data are presented as mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA.