Figure 4.

Inhibition of autophagy during CS sensitization alleviates the neutrophilic airway inflammation in vivo. (A) experimental outline and protocol for 3-MA treatment, 3-MA (4 mg/ml, 50 μl) was administrated intratracheally 2 hrs before each indicated CS exposure day. (B) Inflammatory cell counts in the BALF. (C–F) The Cxcl1, Cxcl2, Il6 and Ifng mRNA transcripts in lungs. Labeling for all the columns throughout Figure 4 was shown in (B). Mac, macrophages; Neu, neutrophils; Lym, lymphocytes; Eos, eosinophils. Throughout, data are representative of 3-5 mice and were replicated in at least 3 independent experiments. Data are presented as mean ± s.e.m. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA.