Figure 5.

Induction of MMP12 by CS during sensitization phase is critical for the ensuing airway inflammation induced by elastin. (A) experimental outline and protocol for doxycycline treatment, mice were fed with doxycycline (2 mg/ml) in drinking water during CS sensitization phase. (B) Expression of Mmp12 mRNA transcripts in mouse BALF cells. (C) Inflammatory cell counts in the BALF. (D–G) The Cxcl1, Cxcl2, Il6 and Ifng mRNA transcripts in lungs. Labeling for all the columns throughout Figure 5 was shown in (C). Mac: macrophages; Neu: neutrophils; Lym: lymphocytes; Eos: eosinophils. Dox: doxycycline. Throughout, data are representative of 4-6 mice and were replicated in at least 3 independent experiments. Data are presented as mean ± s.e.m. Difference between two groups was identified using the Student t-test (B) and multiple groups using one-way ANOVA (C–G). *p < 0.05, **p < 0.01, ***p < 0.001.