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. 2021 Mar 22;12:661357. doi: 10.3389/fimmu.2021.661357

Figure 5.

Figure 5

IDO1 inhibitor-loaded nanosheets can mitigate the inhibitory effects of tryptophan metabolites. (A) KYN production of EC1 cells cultured with or without IDO1 inhibitor, measured via ELISA. (B) Effects of nanosheets on ESCC EC1 cell proliferation; (C) CAR-T-mediated killing of tumor cells. EC1 cells carrying the firefly luciferase gene were cocultured with CAR-T cells at different ratios, after 24 h of culture with or without IDO1 inhibitor. Luminescence was used to determine percentage tumor cell death. (D) CD107a expression of CAR-T cells cocultured with EC1 cells at different E:T ratios for 6 h. The EC1 cells were first cultured with or without IDO1 inhibitor for 24 h. (E) Cytokine production of CAR-T cells cocultured with EC1 cells with or without IDO1 inhibitor. IFN-γ and IL-2 secretion of CAR-T cells, detected via ELISA. *P < 0.05, **P < 0.01, ***P < 0.001, ns, Not statistically significant (repeated-measures one-way ANOVA or Student’s t-test).