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. 2021 Mar 22;15:646468. doi: 10.3389/fnins.2021.646468

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Copyright © 2021 Shiromani, Blanco-Centurion and Vidal-Ortiz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neurons containing the calcium indicator, GCaMP6, relative to the focal plane of the GRIN lens. The top figure schematically depicts that a microinjection of AAVDJ-EF1a-DIO-GCaMP6m is made to express the calcium indicator, GCaMP6 in neurons that are cre positive. At the same time a GRIN lens (600 μm diameter) is inserted at the injection site and electrodes to record the EEG and EMG are implanted. Three weeks after injection, a miniscope is attached to image the fluorescence associated with the intracellular changes in calcium. Bottom figure is the postmortem histology depicting many GCaMP6 containing neurons below the GRIN lens.