TABLE 2.
Primary/secondary antibodies and respective dilution used for immunofluorescence experimental procedures.
| Antigen | Primary antibody | Dilution in PBS | Secondary antibody | Dilution in PBS |
| Caspase-3 | Rabbit monoclonal anti-caspase-3 (Cell Signaling Technology, Danvers, United States) | 1:200 | Alexa 594-conjugated anti-rabbit antibody (Alexa Fluor, Molecular Probes, Invitrogen) | 1:200 |
| Caspase-8 | Rabbit monoclonal anti-caspase-8 (Cell Signaling Technology, Danvers, United States) | 1:100 | Alexa 594-conjugated anti-rabbit antibody (Alexa Fluor, Molecular Probes, Invitrogen) | 1:200 |
| PARP-1 | Rabbit monoclonal anti-PARP-1 (Cell Signaling Technology, Danvers, United States) | 1:200 | Alexa 594-conjugated anti-rabbit antibody (Alexa Fluor, Molecular Probes, Invitrogen) | 1:200 |
| RIP1 | Rabbit polyclonal anti-RIP1 (Santa Cruz Biotechnology) | 1:200 | Alexa 594-conjugated anti-rabbit antibody (Alexa Fluor, Molecular Probes, Invitrogen) | 1:200 |
| MLKL | Mouse monoclonal Anti-MLKL Antibody, clone 3H1 (Sigma-Aldrich) | 1:200 | Alexa 594-conjugated anti-mouse antibody (Alexa Fluor, Molecular Probes, Invitrogen) | 1:200 |
| LC3B | Rabbit polyclonal anti-LC3B (Cell Signaling Technology, Danvers, United States) | 1:400 | Alexa 594-conjugated anti-rabbit antibody (Alexa Fluor, Molecular Probes, Invitrogen) | 1:200 |
| p62/SQSTM1 | Mouse monoclonal anti- p62/SQSTM1 (Abcam, Cambridge, United States) | 1:100 | Alexa 488-conjugated anti-mouse antibody (Alexa Fluor, Molecular Probes, Invitrogen) | 1:200 |
| AIF | Rabbit polyclonal anti-AIF (Cell Signaling Technology, Danvers, United States) | 1:200 | Alexa 594-conjugated anti-rabbit antibody (Alexa Fluor, Molecular Probes, Invitrogen) | 1:200 |
| Golgi | Human autoimmune serum recognizing proteins of Golgi Apparatusa | 1:200 | Alexa 594-conjugated anti-human antibody (Alexa Fluor, Molecular Probes, Invitrogen) | 1:200 |
| Mitochondria | Human autoimmune serum recognizing the 70 kDa E2 subunit of the pyruvate dehydrogenase complexb | 1:200 | Alexa 594-conjugated anti-human antibody (Alexa Fluor, Molecular Probes, Invitrogen) | 1:200 |
| α-tubulin | Mouse monoclonal anti-α-tubulin (Cell Signaling Technology, Danvers, United States) | 1:1000 | Alexa 488-conjugated anti-mouse antibody (Alexa Fluor, Molecular Probes, Invitrogen) | 1:200 |
| Actin | Alexa 488-Phalloidin/Alexa 594-Phalloidin (Molecular Probes, Invitrogen) | 1:500 | — | — |