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. 2021 Mar 22;9:640957. doi: 10.3389/fcell.2021.640957

FIGURE 3.

FIGURE 3

Effect of cabozantinib combined with topotecan on the expression of ABCG2 and topoisomerase I (TOP1). Expression of ABCG2 protein (A) and TOP1 protein (B) in H460 and H460/TPT10 cell lines treated for 72 h with vehicle control, topotecan (TPT) 30 nM, cabozantinib (CBZ) 5 μM, and combination of topotecan and cabozantinib, respectively, followed by the quantitative analysis. For ABCG2 expression, an imaged obtained with extended exposure time was shown as evidence for the endogenous ABCG2 expression in NCI-H460 cells and was not for quantitative use. The relative protein expression was calculated based on the ratio of target protein versus the loading control protein GAPDH. Columns and error bars represented average values with SD from three independent measurements. ∗indicates p < 0.05 comparing resistant cell line to parental cell line with the same treatment; #indicates p < 0.05 comparing treatment group to control group of the corresponding cell line.