Table 1.
Antioxidant performance and total phenolic contents (TPC) of petroleum ether (PE), dichloromethane (DCM), ethyl acetate (EA), ethanol (EtOH) and ethanol/water (EtOH/Wt) extracts and different fractions (A-G) of the ethanol/water extract of A. turanica
| Sample | Extraction/ fractionation yield (g) |
EC
50
(µg/ mL)
|
TPC
(mg GAE /g) |
||
|---|---|---|---|---|---|
| DPPH assay | FIC assay | BCB assay | |||
| PE | 5.51 | 355.74 ±132.85 | n.s. | 53.24 ± 2.93 | 43.64 ± 14.20 |
| DCM | 24.23 | 463.49 ± 117.26 | 585.11 ± 16.12 | 28.42 ±0.27 | 75.76 ± 11.22 |
| EA | 1.21 | 101.53 ± 15.95 | 916.34 ± 49.84 | 22.13 ± 6.03 | 106.67 ± 1.39 |
| EtOH | 7.78 | 18.31 ± 0.59 | 905.19 ± 340.13 | 11.29 ± 1.29 | 154.42 ± 3.03 |
| EtOH/Wt | 37.50 | 20.13 ± 1.07 | 47.88 ± 4.72 | 8.14 ± 1.92 | 136.00 ± 3.47 |
| Fr. A | 1.86 | 113.79 ± 16.17 | 47.92 ± 19.35 | 81.43 ± 13.67 | 24.36 ± 1.55 |
| Fr. B | 1.19 | 50.90 ± 4.85 | 28.96 ± 3.23 | 98.17 ± 0.17 | 77.58 ± 0.95 |
| Fr.C | 0.82 | 46.77 ± 4.94 | 65.86 ± 15.59 | 65.90 ± 1.83 | 98.94 ± 0.26 |
| Fr. D | 3.01 | 21.99 ± 1.82 | 118.36 ± 9.49 | 4.92 ± 1.11 | 255.00 ± 10.29 |
| Fr. E | 3.79 | 20.30 ± 3.34 | 53.33 ± 4.83 | 23.83 ± 0.07 | 169.85 ± 4.12 |
| Fr. F | 2.42 | 18.43 ± 0.45 | 80.91 ± 4.32 | 15.19± 3.41 | 148.48 ± 4.10 |
| Fr. G | 1.04 | 37.41 ± 1.74 | 194.76± 26.41 | 16.88 ± 2.54 | 104.24 ± 1.60 |
| BHT | --- | 4.96 ± 0.66 | --- | 0.469 ± 0.22 | --- |
| Vit C | --- | 4.74±0.19 | --- | --- | --- |
| EDTA | --- | --- | 18.94 ± 2.88 | --- | --- |
| Quercetin | --- | --- | 88.35 ± 4.09 | --- | --- |
DPPH: 1, 1-diphenyl-2-picrylhydrazyl.
FIC: ferrous ion chelating.
BCB: β-carotene bleaching.
GAE: gallic acid equivalents.