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. 2021 Mar 22;11:645757. doi: 10.3389/fonc.2021.645757

Figure 1.

Figure 1

PneumaCult™-ALI [PC] medium maintains lung architecture during ex vivo growth. (A) Percentage of viable EwS cells cultured in B- and PC-medium relative to standard culture conditions for 96 h. Values reported represent the mean ± standard deviation (SD) of at least three independent experiments. Statistical analysis of differences between pairs was performed using a two-way ANOVA with a Tukey's post-hoc test (*p < 0.01). (B) H&E sections of NOD-SCID and NSG mouse lungs showing integrity of lung structures after 7 and 14 days. (C) Immunohistochemistry showing CD99 immunoreactive A673 and (D) TC32 cells in NOD-SCID and NSG mice lung slices at 0, 7, and 14 days post-injection. Three animals were included per group (n = 3) and a minimum of 10 lung tissue sections were analyzed per animal. Scale bars = 100 μm.