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. 2021 Mar 22;11:645757. doi: 10.3389/fonc.2021.645757

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Copyright © 2021 Scopim-Ribeiro, Lizardo, Zhang, Dhez, Hughes and Sorensen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of NK cells on ex vivo and in vitro EwS cell growth. (A) Granzyme B and CD99 co-staining in NOD-SCID and NSG mice at 3-days post-injection of EwS cells. Three animals were included per group (n = 3) and a minimum of 10 lung tissue sections were analyzed per animal. (B) NK92 cytotoxic effects on EwS compared to OS cells. Ratio-dependent cytotoxicity of NK92 cells was determined for A673, TC32, and MG63.3 cells. Bar graphs represent the mean ± SD of a minimum of three independent experiments. Statistical analysis of differences was calculated using a Student's t-test (*p < 0.01).