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. 2021 Mar 22;12:643011. doi: 10.3389/fpls.2021.643011

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Copyright © 2021 Li, Liao, Mei, Pan, Zhang, Zheng, Xie and Miao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis of five OsWRKY genes and the defense-related marker gene OsNAC4 in response to M. oryzae infection. qRT-PCR analysis of five OsWRKYs and OsNAC4 in WT at 0, 24, 48, 72, 96, and 108 h after pathogen treatment. The Y-axis represents the relative expression level normalized to OsACTIN. Box-and-whisker plots show median value (line within box), interquartile range (boxes), and minimum and maximum values (whiskers). Three biological replicates and three technique replicates were used. Asterisk indicate significant differences (**P < 0.01, and ***P < 0.001) based on Student t-test compared to 0 h.