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. 2021 Mar 22;12:643011. doi: 10.3389/fpls.2021.643011

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Copyright © 2021 Li, Liao, Mei, Pan, Zhang, Zheng, Xie and Miao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotyping of detached leaves after darkness treatment. (A) A delaying leaf senescence shown in the OsWRKY93_V_P₆₄ (OsW93_v_p₆₄) compared to Kitaake plants. (B) An early leaf senescence shown in the oswrky93-1 compared to Nipponbare (NIP) plants. Detached leaf pieces of rice were incubated with 1 μM MES (pH8.5) buffer after darkness treatment for 0, 48, 72, and 84 h.