Fig. 5.

Cholesterol inhibited TMEM16A currents in HAECs. A. The time course of TMEM16A currents at +100 mV activated by 25 μM Ca²⁺ in HAECs before (a) and after (b) cholesterol (20 μM) application. Cells were clamped from ramps from −100 to +100 mV with a 750-ms duration at 10 s intervals. The currents were normalized to peak current before cholesterol application. B. Representative currents recorded before (a) and after (b) cholesterol application in A. C. Representative currents before (a) and after (b) cholesterol treatment (20 μM). Currents were elicited with 750-ms voltage step from –100 mV to +100 mV in 20 mV increments. D. The normalized currents (I_normalized) before and after cholesterol application. n = 4 cells.*p < 0.05 vs before cholesterol application.