Table 2.
CRISPR/Cas-based crop improvement with resistance to virus, bacterial and fungal diseases.
| Crop | Targeted gene | Disease resistance | Results | CRISPR/Cas system | Transformation method | Type 0f mutation | Reference |
|---|---|---|---|---|---|---|---|
| Watermelon | Clpsk1 | Fungus | Resistance to Fusarium oxysporum f.sp. niveum | CRISPR/Cas9 | Agrobacterium | Stable | [83] |
| Arabidopsis | eIF(iso)4E | potyvirus | Resistance to Turnip mosaic virus (TuMV) | CRISPR/Cas9 | Floral dip | Stable | [87] |
| Rice | Os8n3 | Bacteria | Resistance to Xanthomonas oryzae pv. oryzae (Xoo) | CRISPR/Cas9 | Agrobacterium | Stable | [90] |
| Rice | OsERF922 | Fungus | Resistance to Magnaporthe oryzae | CRISPR/Cas9 | Agrobacterium | Stable | [85] |
| Tomato | SlJAZ2 | Bacteria | Resistance to Pseudomonas syringae pv. tomato (Pto) DC3000 | CRISPR/Cas9 | Agrobacterium | Stable | [93] |
| Citrus | CsLOB1 | Bacteria | Resistance to Xanthomonas citri subsp. citri (Xcc) | CRISPR/Cas9 | Agrobacterium | Stable | [91] |
| Orange | CsWRKY22 | Bacteria | Xanthomonas citri subsp. citri (Xcc) | CRISPR/Cas9 | Agrobacterium | Stable | [92] |
| Cucumber | eif4e | Virus | Resistance to Cucumber vein yellowing virus, Zucchini yellow mosaic virus and Papaya ring spot mosaic virus‐W | CRISPR/Cas9 | Agrobacterium | Stable | [88] |
| Cotton | 14–3-3 | Fungus | Resistance to Verticillium dahliae | CRISPR/Cas9 | Agrobacterium | Stable | [86] |
| Wheat | mlo | Fungus | Resistance to powdery mildew | CRISPR/Cas9 | Gene gun and protoplast | Stable | [80] |
| Tomato | SiMlo | Fungus | Resistance to powdery mildew | CRISPR/Cas9 | Agrobacterium | Stable | [81] |