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. 2021 Mar 18;45(5):67. doi: 10.3892/or.2021.8018

Figure 6.

Figure 6.

TGFB2 overexpression counteracts the miR-454-3p-mediated suppressive effects on NSCLC cell proliferation. (A and B) A549 and NCI-H1650 cells were co-transfected with TGFB2-overexpressing plasmid and miR-454-3p mimics and then the expression of TGFB2 was determined by RT-qPCR assay. (C-F) The effect of miR-454-3p and TGFB2 on the proliferation of A549 and NCI-H1650 cells was examined by (C and D) CCK-8 assay and (E and F) EdU cell proliferation assay. Scale bar, 100 µm. Data are presented as the mean ± SD, n=3. **P<0.01 and ***P<0.001 compared with cells transfected with the NC + Vector group. #P<0.05 and ##P<0.01 compared with cells transfected with miR-454-3p mimics. TGFB2, transforming growth factor-β2; miR, microRNA; NSCLC, non-small cell lung cancer; RT-qPCR, reverse transcription-quantitative PCR; CCK-8, Cell Counting Kit-8; EdU, 5-ethynyl-2-deoxyuridine; NC, negative control mimics; miR-454-3p, miR-454-3p mimics.