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. 2021 Mar 18;45(5):67. doi: 10.3892/or.2021.8018

Figure 7.

Figure 7.

TGFB2 reverses miR-454-3p-mediated inhibition of migration, invasion and EMT in NSCLC cells. A549 and NCI-H1650 cells were transfected with TGFB2-overexpressing plasmid and miR-454-3p mimics. (A and B) A wound healing assay was performed to evaluate cell migration capacity. Scale bar, 200 µm. (C and D) Transwell invasion assay was conducted to investigate cell invasion capacity. Scale bar, 200 µm. (E and F) Western blot analysis was used to reveal the expression of E-cadherin and N-cadherin in cells after the indicated transfections. Data are presented as the mean ± SD, n=3. *P<0.05, **P<0.01 and ***P<0.001 vs. cells transfected with the NC + Vector group. #P<0.05 and ##P<0.01 vs. cells transfected with miR-454-3p mimics. TGFB2, transforming growth factor-β2; miR, microRNA; EMT, epithelial-mesenchymal transition; NSCLC, non-small cell lung cancer; NC, negative control mimics; miR-454-3p, miR-454-3p mimics; ns, no significance.