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. 2021 Feb 24;13:1759091421991769. doi: 10.1177/1759091421991769

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — DNA Methylation of Autophagy Genes Is Increased Post-Stroke. DNA methylation of Beclin-1 was significantly increased in both the (A) contralateral (F_{2, 26} = 81.77, p < 0.0001) and (B) ipsilateral (F_{2, 31} = 21.76, p < 0.0001) hemispheres of both the saline and LPS exposed stroke animals, when compared to the sham controls. Similarly, DNA methylation of ATG5 was significantly increased in both the (C) contralateral (F_{2, 26} = 322.5, p < 0.0001) and (D) ipsilateral (F_{2, 31} = 53.58, p < 0.0001) hemispheres of both the saline and LPS exposed stroke animals, when compared to the sham controls. DNA methylation of LC3a was significantly increased in the (E) contralateral (F_{2, 26} = 140.4, p < 0.0001) hemisphere of both saline and LPS exposed stroke animals, while it was significantly upregulated in only the (F) ipsilateral (F_{2, 31} = 19.32, p < 0.0001) hemispheres LPS exposed stroke animals, when compared to the sham controls. DNA methylation of LC3b was also significantly increased in the (G) contralateral (F_{2, 26} = 229.7, p < 0.0001) hemisphere of both saline and LPS exposed stroke animals, while it was significantly upregulated in only the (H) ipsilateral (F_{2, 31} = 21.45, p < 0.0001) hemispheres LPS exposed stroke animals, when compared to the sham controls. For all assessed genes, LPS exposed stroke animals had significantly higher levels of DNA methylation than the saline exposed stroke animals (A-H). Data are expressed as mean ± SEM; one-way ANOVA with Tukey’s post hoc test; brackets connect statistically significant groups; *p < 0.05, **p < 0.01, **p < 0.001, and ****p < 0.0001.