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. 2020 Oct 9;137(13):1754–1764. doi: 10.1182/blood.2020006334

Figure 6.

Figure 6.

GCK kinase activity is required for MM cell growth. (A) GCK consists of 820 amino acid residues. The kinase domain (in orange) is located at its N terminus. Lysine 45 is critical for GCK kinase activity. Lysine 45 was replaced by alanine for kinase dead mutation. (B-F) MM.1S cells were transduced with EV (PCDH-EV), shRNA-resistant GCK-M5 (PCDH-GCK(M5)-myc), or shRNA-resistant GCK and GCK kinase dead mutation K45A-M5 (PCDH-GCK-K45A(M5)-myc). GFP sorted cells were cultured in the presence of Dox and analyzed for (B) GCK expression by western blot, (C) proliferation by MTS assay, and (D) for GCK and c-MYC expression by western blot. Selected cells from panel D were cultured in the presence of Dox and analyzed for (E) cell proliferation by MTS assay (F) apoptosis by Annexin V and 7-AAD staining.