Figure 4.

Ingenuity pathway analysis of the TREM1 signaling pathway in the DH82 cell line treated with B. canis. (A) TREM1 signaling pathway in 24 h on coculture model, (B) TREM1 and TLR-associated pathway on TREM1 signaling pathway, (B) is enlarged from rectangle of (A) genes with upregulation are shown in red, and genes with downregulation are shown in green. Orange indicates the predicted activated genes, blue indicates the predicted inhibited genes, and an uncolored node indicates that the genes were not differentially expressed in this pathway. The IPAs were produced using QIAGEN's IPA (https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis). Both the models have been identified in this pathway, particularly on the pathways of TREM1 signaling with association to TLR, increasing over time.