Figure 3.

Triple luciferase imaging in vivo

Triple luciferase imaging in the same animal using 3 independent reporter-substrate combinations. All the animals bear a xenograft derived from 1:1:1 mixed C6 cells with CBRLuc, maCLuc, and RLuc reporter genes on the right shoulder. (A–C) On the left shoulder, animals had xenografts derived from a single C6 line transduced with the FLuc (A), maCLuc (B), or RLuc (C) reporter. Three different luciferase substrates (d-luciferin, vargulin, and coelenterazine; vertical columns) were injected i.v. into each animal (A–C), and imaging was performed sequentially with a 4- to 12-h interval between sessions (n = 5 for each group).