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. 2021 Mar 17;21:15–22. doi: 10.1016/j.omto.2021.03.004

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© 2021 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Imaging of secondary tumor development

Three weeks after administration of bone-avid MDA-MD-231/831 (FLuc/GFP-maCLuc/mCherry) and lung-avid MDA-MB-231/4175 (FLuc/GFP-RLuc/mTurquoise) human breast cancer cell lines, BLI images (middle panel) were obtained using three independent substrates: vargulin, d-luciferin, and coelenterazine, which are specific for maCLuc, FLuc, and RLuc, respectively. Immunofluorescence analysis of an MDA-231-831 bone metastasis (GFP/mCherry, left panel) and an MDA-231-4175 lung metastasis (GFP/mTurquoise, right panel). Each experiment was repeated three times (n = 5 for each group).