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Representative cases of (A) perihilar or (B) distal bile duct carcinoma showing H&E staining, MSLN and CA125 expression. Cases 1 and 4 represent MSLN and CA125 high-level expression. Cases 2 and 5 represent MSLN high-level expression and CA125 low-level expression. Cases 3 and 6 represent MSLN low-level expression and CA125 high-level expression. Magnification, ×200. H&E, hematoxylin and eosin; MSLN, mesothelin; CA125, cancer antigen 125.
