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. 2021 Mar 26;21(5):416. doi: 10.3892/ol.2021.12677

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Copyright: © Ren et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — Expression of PDK4 was regulated by circCCDC66 via sponging miR-211-5p in thyroid cancer cells. (A) Compared with the control group, PDK4 expression level in circCCDC66 knockdown cells was decreased. (B) Schematic diagram of the wild-type or mutant-type binding sequence predicted on the online website. (C) Expression level of miR-211-5p was significantly upregulated in CAL62 and TPC1 cells following transfection with miR-211-5p-mimic. (D) Compared with the control group, PDK4 expression level was lower in cells overexpressing miR-211-5p. (E) Expression of circCCDC66 in TPC1 and CAL62 cells following transfection with miR-211-5p-mimic was decreased compared with control group. (F and G) miR-211-5p-mimic reduced the activity of circCCDC66 and PDK4 plasmid-transfected TPC1 and CAL62 cells according to results from luciferase reporter assay. (H and I) miR-211-5p-mimic had little effect on the activity of circCCDC66-MUT and PDK4-MUT plasmid-transfected TPC1 and CAL62 cells according to results from luciferase reporter assay. *P<0.05 and **P<0.01. MUT, mutant; miR, microRNA; PDK4, pyruvate dehydrogenase kinase 4.