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. 2021 Mar 30;14:2247–2258. doi: 10.2147/OTT.S294129

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© 2021 Lu et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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BCG treatment recruits infiltrating lymphocytes in the TME of TNBC mouse model.

Notes: (A) Schematic of BCG treatment on TNBC mouse model. n = 5 mice for PBS, 4 mice for BCG. (B–E) Representative flow cytometry plots for 4T1 tumor-infiltrating CD3⁺ (B), CD4⁺ (C), CD8⁺ (D), NK (E) cells analysis. (F) Quantification of the tumor-infiltrating lymphocytes from TNBC mouse model. n = 5 mice for PBS, 4 mice for BCG. Mean ± s.e.m., two-tailed unpaired Welch’s t-test. (G and H) Fluorescence images of CD3-PE-stained 4T1 tumor cells treated with BCG and PBS. Representative images of each group (n = 3 mice) are presented. Scale bar, 20 μm.

Abbreviation: BCG, Bacillus Calmette–Guérin.