FIG. 3.

HDCA inhibits TRAP1 but not Hsp90 ATPase activity. (A) Spectrophotometric quantification of the effect of HDCA on the ATPase activity of purified human TRAP1 or Hsp90α proteins (blue and orange bars, respectively). Wide-spectrum Hsp90 family inhibitors radicicol and 17-AAG were used as positive controls. (B) Dose-response analysis of the effect of HDCA on TRAP1 ATPase activity. Throughout the figure, data are presented as mean ± SEM (n = 4); ***p < 0.001 with an unpaired two-tailed Student's t test. 17-AAG, 17-N-allylamino-17-demethoxygeldanamycin; Hsp90, heat shock protein 90; SEM, standard error of the mean.