FIG. 5.

HDCA reverts TRAP1-dependent inhibition of SDH in a concentration-dependent and reversible way. (A) Dose-response analysis of a 6-h cell exposure to the reported concentrations of HDCA. (B) Effect of a 6-h exposure of sMPNST cells to 5 μM HDCA (on) followed by compound withdrawal from medium (off) for the reported times. Data are shown as mean ± SEM (n > 3); ***p < 0.001; *p < 0.05 with one-way ANOVA with post hoc Bonferroni's test. Spectrophotometric assessments of SQR activity of SDH were carried out as in Figure 4. Cells are labeled as in Figure 4.