FIG. 7.

Effect of TRAP1 inhibition on in vitro tumorigenesis. (A) Representative images of focus-forming assays performed on sMPNST cells grown for 9 days in the presence of the indicated concentrations of HDCA. (B) Bar graph quantification of the effect of TRAP1 inhibition, either by CRISPR/Cas9 knock-out or by administration of the reported concentrations of HDCA, on the growth of foci. Data are reported as mean ± SEM (n = 3); ***p < 0.001 with one-way ANOVA with post hoc Bonferroni's test. (C) Representative images of foci after 6 or 9 days of assay. Where indicated, 5 μM HDCA was added at the sixth experimental day and kept in the medium for the next 3 days. (D) Bar graph quantification of the effect of HDCA (5 μM, added as in C) on the growth of foci. Data are reported as mean ± SEM (n = 3); ^§§§p < 0.001 with an unpaired two-tailed Student's t test for the values obtained after 6 days of assay; ***p < 0.001 against basal values at the ninth experimental day with one-way ANOVA with post hoc Bonferroni's test. In (B, D), foci were quantified by using an integrated density parameter that englobes both their thickness and area. Cells are labeled as in Figure 4.