FIG. 8.

Effect of HDCA on the SDH activity of SIRT3-overexpressing cells. (A) Western immunoblot showing SIRT3, TRAP1, and SDHA protein expression levels in sMPNST cells transfected either with a control pFUGW-GFP vector or with a pFUGW-SIRT3. β-actin was used as a protein loading control. (B) Spectrophotometric assessment of the SQR activity of SDH on cells treated with HDCA (5 μM, 6 h). Data are shown as mean ± SEM (n > 3); ***p < 0.001 against pFUGW-GFP-transfected cells; ^§§§p < 0.001 against HDCA-treated GFP cells with one-way ANOVA with post hoc Bonferroni's test. (C) Model of the HDCA effects on TRAP1 and SIRT3. SIRT3, sirtuin-3.