FIG. 3.

Human sensory neurons and keratinocytes cultured in a microfluidic chamber with microgrooves. (A) Microfluidic chamber (approximate 2 cm square) made of PDMS. (B) Illustration of microfluidic chamber with keratinocytes in the flow-through compartment (arrow indicates flow direction) and sensory neurons in the apposed compartment separated by microgrooved channels. (C) When neurons are incubated with the lipophilic, crystalline red dye, DiI, axons (arrows) can be observed crossing the microgrooved channels to the keratinocyte side. Scale bar = 50 μm. PDMS, polydimethylsiloxane. Color images are available online.