FIG. 3.

Antigenic interactions of Protoparvoviruses. (A) Capsid surface representation of MVM. The approximate locations of the 2-, 3-, and 5-fold axes are indicated along with the 2/5-fold wall and 3-fold protrusions. (B) Structural superimposition of VP monomers from different Protoparvoviruses. The VRs are labeled. (C) Depiction of the cryo-reconstructed density maps of the MVM, CPV, and FPV viral capsids complexed to the Fab portion of monoclonal antibodies. The viral strains are identified in the capsid wheel located to the left of the figure. Generic or specific Fabs are docked onto the viral capsids at the appropriate locations identified by the cryo-reconstructed maps. These epitopes span the 2-fold, 3-fold, and 5-fold axes together with the 2/5-fold wall. The viral capsids in (A, B) are colored radially, such that regions closest to the center are colored purple, and those farthest away are colored red, with the Fabs colored gray, as indicated by the scale bar (bottom right in Fig. 1). These images were generated using PyMol (31). CPV, canine parvovirus; FPV, feline panleukopenia virus; MVM, minute virus of mice immunosuppressive strain. Color images are available online.