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. 2021 Feb 12;18(1):20–28. doi: 10.1089/zeb.2020.1951

FIG. 4.

FIG. 4.

The stable transgenic line Tg(sox17:TAEL-N) restricts light-induced expression to endoderm-derived tissues. (A) Schematic depicting experimental design. Tg(sox17:TAEL-N) and Tg(C120F:GFP) adult zebrafish were crossed to produce double transgenic embryos. GFP expression was activated by illuminating embryos for 12 h, starting at 6 hpf, with blue light pulsed at a frequency of 1 h on/1 h off. (B–E) Representative images of Tg(sox17:TAEL-N);Tg(C120F:GFP) embryos exposed to blue light (B, C) or kept in the dark (D, E). Images were acquired between 18 and 20 h postactivation. Arrow in (B) indicates gut tube (GT). Dashed lines in (B) indicate derivatives of the DFCs. Arrowhead in (C) indicates PE. (B, D) are lateral views, anterior to the left. (C, E) are dorsal views, anterior to the left. Scale bars, 200 μm. DFCs, dorsal forerunner cells; PE, pharyngeal endoderm.