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. 2021 Mar 22;118(13):e2021385118. doi: 10.1073/pnas.2021385118

Fig. 6.

Fig. 6.

T-Uqcrfs1−/− iNKT cells have impaired TCR signaling and altered responsiveness to IL-15. (A) Expression of Nur77 in CD4sp, CD8sp, and DP thymocytes. (B) Bar graphs depict mean + SEM of Nur77 mean fluorescence intensity (MFI) (n = 6). (C) Representative recordings of Ca2+ flux in WT and T-Uqcrfs1−/− DP thymocytes measured before and after anti-CD3/anti-CD4 cross-linking in Ca2+ free DPBS, and after addition of 2 mM Ca2+ in DPBS. (D and E) Peak of calcium flux (ratios of Fluo 4/Flura red) after anti-CD3 cross-linking (D) and Ca2+ addition (E) (n = 5). (F) Expression of T-bet in thymic iNKT cells. (G) Bar graphs depict mean + SEM of T-bet MFI (n = 6). (H) Representative histograms show CD122 expression on thymic iNKT cells. (I) Bar graphs depict mean + SEM of CD122 MFI (n = 7). (J and K) Thymocytes were incubated in the presence or absence of IL-15. Surface staining with CD1d/PBS57 tetramer and anti–TCR-β were performed, followed by intracellular staining for pSTAT5. (J) Representative histograms show pSTAT5 level in iNKT cells. (K) Bar graphs depict mean + SEM of pSTAT5 MFI (n = 5). Data are representative of three experiments. *P < 0.05, **P < 0. 01, and ***P < 0.001.