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. 2021 Mar 22;118(13):e2100194118. doi: 10.1073/pnas.2100194118

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Copyright © 2021 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).

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Fig. 2. — pS6 expression after leptin treatment of ob mice. Dual ISH and IHC in hypothalamus and brainstem was performed for the marker genes and pS6 (244 and 247) as indicated in sections from ob mice treated with leptin (14 d) vs. PBS. (A) POMC: ISH for Pomc and IHC for pS6 in arcuate nucleus. Pomc neurons in Arc are activated by 14 d leptin treatment of ob/ob mice. (B) Tph2: ISH for Tph2 and IHC for pS6 in dorsal raphe. Tph2 neurons in dorsal raphe are activated by 14 d leptin treatment of ob/ob mice. (C) CRH: ISH for Crh and IHC for pS6 in inferior colliculus. Crh neurons in inferior colliculus are activated by 14 d leptin treatment of ob/ob mice. (D) Ppp1r17: ISH for Ppp1r17 and IHC for pS6 in DMH. Ppp1r17 neurons in DMH are inhibited by 14 d leptin treatment of ob/ob mice. (Scale bars, 50 μm.)