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. 2021 Mar 22;118(13):e2100194118. doi: 10.1073/pnas.2100194118

Fig. 3.

Fig. 3.

Effect of chemogenetic modulation of DMHPpp1r17 neurons on food intake and body weight in WT mice. (A) Chemogenetic activation of DMHPpp1r17 neurons using AAV8-hSyn-DIO-hM3D(Gq)-mCherry. DREADD-induced activation of DMHPpp1r17 neurons significantly decreases food intake over the course of 4 h. (B) Chemogenetic inhibition of DMHPpp1r17 neurons using AAV8-hSyn-DIO-hM4D(Gi)-mCherry. DREADD-induced inhibition of DMHPpp1r17 neurons does not affect food intake over the course of 4 h. (C) Body weight of Ppp1r17-Cre mice is significantly reduced after chronic activation of DMHPpp1r17 neurons by i.p. injection of CNO two times per day. (D) Body weight of Ppp1r17-Cre mice does not significantly change after chronic inhibition of DMHPpp1r17 neurons by i.p. injection of CNO two times per day (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, two-tailed unpaired t test; n = 5 to 6 mice). All error bars are mean ± SD.