Fig. 6.
cFos expression in Ppp1r17 neurons in ob mice and during scheduled feeding. (A) IHC for Ppp1r17 and TdTomato in ObRb TdTomato mice. DMHPpp1r17 neurons do not express Leprb. (B) cFos expression in DMHPpp1r17 neurons in ob/ob mice is reduced by 14 d pair-feeding. (C) cFos expression in DMHPpp1r17 neurons in scheduled feeding during the 3-h feeding window in response to different amounts of chow. Food is provided to mice between Circadian Time (CT)4 and CT7: (Upper) 4 g of food, the amount normally consumed during the 3-h feeding window; (Middle) 2 g of food, the amount mice consume in 3 h after a fast; and (Lower) 1 g, the amount consumed during the dark phase within 3 h. (Scale bars, 50 μm.)