Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Mar 30;220(6):e202002084. doi: 10.1083/jcb.202002084

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Kulkarni et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 2. — Synaptic activity regulates AV motility in dendrites. (A) Quantitation of mean Fluo-4 fluorescence intensity in rat hippocampal neurons (15 DIV) before and after on-scope addition of 4-AP + Bic or DMSO. (B) Quantitation of mean Fluo-4 fluorescence intensity in rat hippocampal neurons (25 DIV) treated with 4-AP + Bic before and after on-scope addition of CNQX + AP5. (C) Mean Fluo-4 intensity in rat hippocampal neurons (14 DIV) either transfected or untransfected with mCherry-LC3 and treated with 4-AP + Bic or CNQX + AP5 for 60 min. (D) Kymographs of mCherry-LC3, tracked AVs (magenta denotes retrograde, cyan denotes anterograde, and dark blue denotes stationary segments), and GCaMP3 from the same dendrite after 30 min of treatment in 4-AP + Bic, DMSO, or CNQX + AP5. Horizontal bar, 5 µm. Vertical bar, 1 min. (E–G) Quantitation of AV mean speed in dendrites of rat hippocampal neurons treated for 30 min in 4-AP + Bic, DMSO, or CNQX + AP5 (E, mean ± SEM; F, histogram of dendritic AV mean speed; G, cumulative frequency of dendritic AV mean speed; n = 629–731 AVs from 26–32 neurons from three to four independent experiments; 13–14 DIV; one-way ANOVA with Tukey’s post hoc test; ***, P ≤ 0.001; ****, P ≤ 0.0001). (H) Quantitation of average dendritic AV mean speed per neuron (mean ± SEM; n = 26–32 neurons from three to four independent experiments; 13–14 DIV; one-way ANOVA with Tukey’s post hoc test; *, P ≤ 0.05; ***, P ≤ 0.001). (I) Quantitation of GCaMP3 fluorescence intensity in the dendrite of a neuron treated with 4-AP + Bic, DMSO, or CNQX + AP5 for 30 min.