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. 2021 Mar 30;220(6):e202002084. doi: 10.1083/jcb.202002084

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© 2021 Kulkarni et al.

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Figure 8. — Synaptic activity increases the density of acidic organelles in dendrites. (A) Straightened images of GCaMP3 and Lysotracker-Red in dendrites from hippocampal neurons treated with 4-AP + Bic, DMSO, or CNQX + AP5 for 30 min. The soma is toward the right. Scale bar, 5 µm. (B and C) Corresponding quantitation of the number of Lysotracker-Red puncta per 10-µm dendrite (B, mean ± SEM; C, cumulative frequency; n = 21–22 neurons from three independent experiments; 13–14 DIV; one-way ANOVA with Tukey’s post hoc test; **, P ≤ 0.01).